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Agarose gel electrophoresis

Essay specific features

 

Issue:

Science

 

Written by:

Frank D

 

Date added:

January 16, 2015

 

Level:

University

 

Grade:

A

 

No of pages / words:

5 / 1167

 

Was viewed:

6484 times

 

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Essay content:

Agarose is a polysaccharide purified from seaweed. An agarose gel is created by suspending dry agarose in a buffer solution, boiling until the solution becomes clear, and then pouring it into a casting tray and allowing it to cool. The result is a flexible gelatin-like slab. During electrophoresis, the gel is submersed in a chamber containing a buffer solution and a positive and negative electrode...
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The result is a flexible gelatin-like slab. During electrophoresis, the gel is submersed in a chamber containing a buffer solution and a positive and negative electrode. The DNA to be analyzed is forced through the pores of the gel by the electrical current. Under an electrical field, DNA will move to the positive electrode (red) and away from the negative electrode (black)...
displayed 300 characters

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