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Effects of Phthalates on Motility behavior of Bull Sperm Cells

Essay specific features

 

Issue:

Miscellaneous

 

Written by:

Jeffrey T

 

Date added:

April 30, 2015

 

Level:

University

 

Grade:

A

 

No of pages / words:

64 / 17750

 

Was viewed:

7763 times

 

Rating of current essay:

 
Essay content:

In the control experiments, the sperm cell suspension was a 1:2 dilution of reactivated bull semen and 300 mosmol HEPES basic medium. While in the impact experiments, HEPES basic medium was changed to HEPES basic plus stress compound. Four different concentrations of Dimethyl Phthalates and Dibutly Phthalates were used as stress compounds...
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Motility parameters were measured under exposure of bull sperm cells to 10-3, 10-4, 10-5and 10-6 molar solutions of DMP and 10-5, 10-6, 10-7 and 10-8 molar solutions of DBP. It was also possible to compare the effects of both DMP and DBP at the same concentrations of 10-5 and 10-6 mol/l DMP and DBP. A Computer Assisted Sperm Analysis (CASA) system was used for measurement of motility parameters relevant to the study such as; Average Path Velocity (VAP), Straight Line Velocity (VSL), Curvilinear Velocity (VCL), Percentage Motility, Amplitude of Lateral Head Displacement (ALH), Head Cross Beat Frequency (BCF) and derived parameters of motility such as Linearity, Straightness and Wobble...
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