Enzme Kinetics

Essay specific features

 

Issue:

Science

 

Written by:

Vivian F

 

Date added:

November 1, 2016

 

Level:

University

 

Grade:

A

 

No of pages / words:

8 / 2141

 

Was viewed:

6531 times

 

Rating of current essay:

 
Essay content:

The rates of an enzyme catalyzed reaction generally changes with change in enzyme concentration, substrate concentration, temperature, pH, time, and presence of enzyme inhibitors. For this particular experiment, the determination of enzyme activity, Trypsin, was obtained by changing the concentration of its substrate concentration (1)...
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For these purposes, the Michaelis-Menten equation was used in combination with a double-reciprocal or Lineweaver-Burke plot to plot obtained data and the enzymatic activity of Trypsin was measured through the colormetric Lowry's assay and spectrophotometer absorbance readings. Results End results of this experiment proved that the changes in substrate concentration are directly proportional to the rate of reaction of an enzyme...
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