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Enzme Kinetics
Essay specific features
Written by:
Vivian F
Date added:
November 1, 2016
Level:
University
Grade:
A
No of pages / words:
8 / 2141
Was viewed:
6531 times
Rating of current essay:
Essay content:
The rates of an enzyme catalyzed reaction generally changes with change in enzyme concentration, substrate concentration, temperature, pH, time, and presence of enzyme inhibitors. For this particular experiment, the determination of enzyme activity, Trypsin, was obtained by changing the concentration of its substrate concentration (1)...
displayed 300 characters
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For these purposes, the Michaelis-Menten equation was used in combination with a double-reciprocal or Lineweaver-Burke plot to plot obtained data and the enzymatic activity of Trypsin was measured through the colormetric Lowry's assay and spectrophotometer absorbance readings.
Results
End results of this experiment proved that the changes in substrate concentration are directly proportional to the rate of reaction of an enzyme...
displayed 300 characters
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