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Essay

Gel Filtration

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Issue:

Science

 

Written by:

Roscoe B

 

Date added:

June 8, 2016

 

Level:

University

 

Grade:

A

 

No of pages / words:

2 / 321

 

Was viewed:

1387 times

 

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Essay content:

This technique can be used particularly in molecular weight determination and on a quantitative analysis of their molecular interactions. “All types of chromatographic methods are based on a very simple principle. The sample to be examined is allowed to interact with two physically distinct entities, a mobile phase and a stationary phase...
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A solution containing solutes of various molecular sizes are allowed to pass through the column under the influence of controlled solvent flow. Solute molecules larger than the pores cannot enter the interior of the gel beads so they are limited to the space between the beads.” (Boyer, 2000) Some common gels or media used in the laboratory today include: the cross-linked dextrans (Sephadex), polyacrylamides (Bio-Gel), dextran-polyacrylamide (Sephacryl) and agarose gels (Sepharose and Bio-Gel A)...
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