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Essay

Green Fluorescent Protein Lab

Essay specific features

 

Issue:

Science

 

Written by:

Jill H

 

Date added:

January 2, 2012

 

Level:

University

 

Grade:

A

 

No of pages / words:

6 / 1623

 

Was viewed:

1194 times

 

Rating of current essay:

 
Essay content:

This lab introduces a rapid method to purify recombinant GFP using HIC. Once the protein is purified, it may be analyzed using polysaccharide gel electrophoresis (PAGE). Purpose: To illustrate the process of transformation and perform it using Green Fluorescent Protein. Experimental Design: This experiment will achieve its purpose by allowing the student to perform a transformation following step by step instructions...
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Mark the sterile 15mL tubes with the correct labelings. (LB+plasmid, LB/Amp+plasmid). 2. Use a sterile transfer pipet to add 250mL of ice cold calcium chloride to each tube. 3. Place both tubes on ice. 4. Use a sterile plastic inoculating tube to transfer isolated colonies of E.coli from the starter plate to the +plasmid tube...
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