Conventional methods for detecting E.coli and coliforms by MacConkey and EMB agar are still practiced in many laboratories. These techniques will take 24 to 48 hours to enumerate and need further confirmatory biochemical tests for identification that will cost time and money. The major disadvantage of using traditional selective/ differential agars such as EMB Agar is that often these media become overgrown with non-E...
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The major disadvantage of using traditional selective/ differential agars such as EMB Agar is that often these media become overgrown with non-E.coli, making it virtually impossible to isolate the E.coli colonies and also giving false negative results. On EMB agar, E.coli is generally observed as green metallic sheen, which may disappear after 48 hours giving false negative results...
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