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Essay

Cell Seeding

Essay specific features

 

Issue:

Science

 

Written by:

Susan R

 

Date added:

November 11, 2016

 

Level:

University

 

Grade:

A

 

No of pages / words:

10 / 2605

 

Was viewed:

8457 times

 

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Essay content:

It previously has been shown that, compared to static seeding conditions, seeding scaffolds under dynamic conditions facilitates high seeding densities and even distributions of cells. The efficiency of seeding HOSTE85 cells and bovine chondrocytes into needled felt scaffolds following agitation at different speeds was determined...
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Seeding efficiency was determined using the Hoechst 33258 assay, and cell viability was assessed using the Alamar Blue™ assay. The distribution of cells within the scaffolds was imaged using scanning electron microscopy. It was found that the optimum seeding conditions varied for HOSTE85 cells and bovine chondrocytes, with different agitation speeds leading to different seeding efficiencies, cell viabilities, and distributions of cells within scaffolds...
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