When the solution that contain isolated guinea-pig ileum and acetylcholine added with atropine, the response obtained is below than the solution isolated guinea-pig ileum and acetylcholine without atropine. It is because agonist will competed with antagonist to bind at the same site of receptor. Antagonists will prevent the agonists to bind and also will prevent the contraction of isolated guinea-pig ileum...
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Antagonists will prevent the agonists to bind and also will prevent the contraction of isolated guinea-pig ileum. But, when we replace the atropine with papaverine, the effect of response is below than the presence of atropine. It is because papaverine is irreversible antagonist. Irreversible antagonist will bind to the active site of receptor, it will not detach from the receptor...
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