The suspension should appear milky white. 6. Return the +plasmid tube to ice. 7. Use a sterile plastic inoculating tube to add one loopful of DNA to the +plasmid tube. immerse the loopful of plasmid DNA directly into the cell suspension and spin the loop to mix the DNA with the cells. 8. Return the +plasmid tube to ice and incubate for 15 minutes...
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Return the +plasmid tube to ice and incubate for 15 minutes. 9. While the tubes are incubating, label your media plates. 10. Following the 15 minute incubation on ice, heat shock the cells using a water bath. 11. Use a sterile transfer pipet to add 250 uL Luria broth to each tube. Gently tap the tubes to mix the LB with the cell suspension...
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