Isolation and Purification of Cholesterol from Egg Yolk

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Issue:

Science

 

Written by:

Keith C

 

Date added:

November 29, 2016

 

Level:

University

 

Grade:

A

 

No of pages / words:

1 / 266

 

Was viewed:

5608 times

 

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Essay content:

The melting point of the yellow, sticky crude product was 91-119 oC. This crude product was then recrystallized from methanol, yielding 0.128g of pale yellow crystals with a melting point of 131-135 oC. This represents 0.33% of the original mass of the two yolks. The cholesterol was then dissolved in ether and further purified by bromination with a bromine/acetic acid reagent and debromination with zinc powder, a series of aqueous washes, and a final recrystallization from methanol...
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This represents 0.33% of the original mass of the two yolks. The cholesterol was then dissolved in ether and further purified by bromination with a bromine/acetic acid reagent and debromination with zinc powder, a series of aqueous washes, and a final recrystallization from methanol. Here, a yield of 28% was recovered from an initial mass of 100 mg of recrystallized material...
displayed 300 characters

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