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Essay

SDSPAGE

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Issue:

Science

 

Written by:

James Y

 

Date added:

November 24, 2013

 

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Grade:

A

 

No of pages / words:

4 / 935

 

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964 times

 

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Essay content:

Before this is done, the sample will be mixed with some of the solution containing SDS (sodium dodecyl sulfate). This compound dissociates in solution yielding the negative large ions that will be useful for the purpose of the electrophoresis. Since the Alkaline Phosphatase still has some positively charged groups on it, even though it has a net negative charge in the loading buffer, the SDS negative ions will surround these positively charged groups and make the protein a large negative mass of a molecule...
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This will be the factor that will guide it through the gel under voltage that will be applied to the gel. There will also be a dye in the sample mixture that is much smaller than the protein samples in the sample mixture. This will give us a reference of when to stop the current and a reference for calculating to the relative mobility of each band that will appear in the gel after electrophoresis...
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