Western Blotting technique laboratory analysis

Essay specific features

 

Issue:

Science

 

Written by:

Tamra B

 

Date added:

December 8, 2016

 

Level:

University

 

Grade:

A

 

No of pages / words:

3 / 808

 

Was viewed:

845 times

 

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Essay content:

Neal Burnette and is a pun on the name Southern blot. Southern blot was a name given by Edwin Southern to a technique he developed for DNA detection. Western blot analysis has the ability to detect one specific protein in a mixture of any number of proteins while at the same time giving you information about the size of the protein...
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It does not matter whether the protein has been synthesized in vivo or in vitro. However, this method does require the use of a high-quality antibody directed against a desired protein. During western blotting procedures, generally, proteins are solubilized with detergents and reducing agents, and then are separated in polyacrylamide gels...
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