Chromatography

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Miscellaneous

 

Written by:

Carolyn D

 

Date added:

April 3, 2015

 

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A

 

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3 / 586

 

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These differences allow the mixture components to be separated from each other by using these differences to determine the transit time of the solutes through a column. Simple liquid chromatography consists of a column with a fritted bottom that holds a stationary phase in equilibrium with a solvent...
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Typical stationary phases (and their interactions with solutes) are: solids (adsorption), ionic groups on a resin (ion-exchange), liquids on an inert solid support (partitioning), and porous inert particles (size exclusion). The mixture to be separated is loaded onto the top of the column followed by more solvent...
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