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Miscellaneous

 

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Loretta F

 

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December 14, 2013

 

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University

 

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B

 

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2 / 448

 

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After high-speed centrifugation, supernatant remained the activity that produced 22-nt guide sequences. RISC, on the other hand, was mostly removed from extracts, suggesting that these two enzyme activities were distinct. The dsRNA specific RNase III family members have several types according to genome analysis of Drosophila and Caenorhabditis elegans...
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Class I has a single RNase III domain and a single DSRNA-binding domain. Drosha, a class 2 example, has two RNAse domains (RIIIa and RIIIb) and a single dsRBD. A third class consisted of two RNase III domains and an amino-terminal helicase domain (CG4792 and CG6493 in Drosophila). S2 cells were transfected with dsRNA and lysed in immunoprecipitation buffer...
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