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Essay

Biotechnology

Essay specific features

 

Issue:

Science

 

Written by:

Rebecca M

 

Date added:

August 20, 2016

 

Level:

University

 

Grade:

A

 

No of pages / words:

5 / 1254

 

Was viewed:

5268 times

 

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Essay content:

After the restriction endonuclease binds, it starts to disrupt, using hydrolysis, the phosphodiester bonds between neighbor nucleotides, causing the H-bonds between base pairs in the cutting region to be broken. This cuts the original double-stranded DNA strand, producing two DNA fragments, which may differ for different restriction endonucleases, depending on where the phosphodiester bond is broken when cut by the endonuclease...
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This process can produce either blunt ends (where ends of the DNA fragment are fully paired with no overhangs), or sticky ends (where both DNA fragments have nucleotides lacking complementary bases and overhangs are produced). However, sticky ends are more useful for genetic engineering. The next step, gel electrophoresis, separates the gene that has been excised, from the unwanted fragments taking advantage of chemical and physical properties of DNA...
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General issues of this essay:
Gene One GENE ONE INC GENE ONE GENE ONE Gene One Gene One Gene One Gene One Gene One Gene One gene one Comparison of a ’true’ fragment to a ’complete’ fragment The Gene Gay Gene Gene One
 

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