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Essay

Determination of a Bacteriophage Titer

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Issue:

Science

 

Written by:

Lonnie S

 

Date added:

June 17, 2017

 

Level:

University

 

Grade:

A

 

No of pages / words:

2 / 314

 

Was viewed:

8394 times

 

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Essay content:

These should be warmed to 37c before use ? 5 tubes of soft agar (0.7% agar). Prior to use, melt and hold at 50c in a water bath ? 5 tubes of 9.0 ml trypticase soy (TS) broth ? 1 ml sterile pipettes ? Pipette aids Methods: 1. We began the experiment by marking the plates and place them in order from 10^5, 10^6, 10^7, then have the blank dilutions ready in order from 10^-4, 10^-5, 10^-6 2...
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The pipettes was used and pipette 0.5 ml of the phage suspension to the 10^4 3. The next step was pipette 0.5 ml of dilution suspension from 10^-4 and added it to the 10^-5 4. 0.5 ml of dilution suspension was pipette from 10^-5 and add it to the 10^-6 5. We then took 0.1 ml from the 10^-6 and add it into the agar...
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