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Determining Optimum Temperature and pH for Enzymatic Reactions of Alpha Amylase

Essay specific features

 

Issue:

Science

 

Written by:

Calvin J

 

Date added:

January 5, 2015

 

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No of pages / words:

8 / 2017

 

Was viewed:

5925 times

 

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Essay content:

In order to determine the optimum temperature for the enzymatic reaction water bath of varying temperatures were made. To determine the ideal pH various buffers were added to the solutions in order to adjust the pH. At scheduled intervals the reacting solution was added to a cuvette containing I2KI indicator in order to stop the reaction, allowing for accurate readings of absorbency...
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Once completed graphs which compared absorbency to temperature and pH were created allowing for a visual reference of the results. Optimum temperature and pH were shown determined by noting where absorbency was the lowest because this is where the enzyme catalyzed the most amount of starch. The results showed that alpha amylase most efficiently converts starch into glucose at a temperature of 65?C and pH of 5...
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