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enzyme function

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Issue:

Science

 

Written by:

Teresa H

 

Date added:

March 5, 2013

 

Level:

University

 

Grade:

A

 

No of pages / words:

5 / 1354

 

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6213 times

 

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Some tissues and cell types (such as erythrocytes), which have no mitochondria and thus cannot oxidize pyruvate to CO2, produce lactate from glucose even under aerobic conditions. The reduction of pyruvate is catalyzed by lactate dehydrogenase (LDH), which forms the L-isomer of lactate at pH=7: CH3-CO-COO- + NADH + H+ ® CH3-CH(OH)-COO- + NAD+ NADH has an absorption maximum at 340 nm...
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So the reaction was followed spectrophotometrically, by observing the decrease in absorbance at 340 nm. In this experiment, the Michaelis constant was determined and the inhibitory effect of oxamate was demonstrated. Procedure Part A: Without inhibitor A series of eight tubes were prepared as shown at table 1: Tubes # 1 2 3 4 5 6 7 8 Imidazole HCl buffer, pH=7...
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