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Identification of unknown plasmid

Essay specific features

 

Issue:

Science

 

Written by:

Mabel R

 

Date added:

September 16, 2014

 

Level:

University

 

Grade:

A

 

No of pages / words:

12 / 3306

 

Was viewed:

5909 times

 

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Essay content:

Abstract Two experiments were done to identify an unknown plasmid. The success of these experiments came from the use of modern day technology involving gel electrophoresis. First, bacterial transformation to E. Coli DH5 was performed on our unknown plasmid along with two known plasmids, pAMP and pKAN, and a negative control TE, a buffer without DNA...
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By performing confluency streaking of bacteria in plates containing antibiotics, we were able to examine the recombinant DNA of the bacteria. After incubation of the plates, we analyzed the samples and found that our unknown plasmid reacted positively on the LB/AMP plate. There were a total growth of three colonies on the LB/AMP plate and a negative result on the LB/KAN plate...
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