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Isolation and Purification of Cholesterol from Egg Yolk
Essay specific features
Written by:
Keith C
Date added:
November 29, 2016
Level:
University
Grade:
A
No of pages / words:
1 / 266
Was viewed:
5669 times
Rating of current essay:
Essay content:
The melting point of the yellow, sticky crude product was 91-119 oC. This crude product was then recrystallized from methanol, yielding 0.128g of pale yellow crystals with a melting point of 131-135 oC. This represents 0.33% of the original mass of the two yolks. The cholesterol was then dissolved in ether and further purified by bromination with a bromine/acetic acid reagent and debromination with zinc powder, a series of aqueous washes, and a final recrystallization from methanol...
displayed 300 characters
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This represents 0.33% of the original mass of the two yolks. The cholesterol was then dissolved in ether and further purified by bromination with a bromine/acetic acid reagent and debromination with zinc powder, a series of aqueous washes, and a final recrystallization from methanol. Here, a yield of 28% was recovered from an initial mass of 100 mg of recrystallized material...
displayed 300 characters
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