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Leading and Lagging strand synthesis in DNA

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Issue:

Science

 

Written by:

Earnest M

 

Date added:

September 5, 2014

 

Level:

University

 

Grade:

A

 

No of pages / words:

1 / 259

 

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9237 times

 

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Essay content:

continuous replication. The difference arises because of the different orientations of the parent template strands. The template of the leading strand is oriented in the 3'>>5' direction (according to the numbering of atoms in the sugar residues), which means that the leading strand itself is oriented in the opposite 5'>>3' direction, providing an -OH group at the 3' end for the continual addition of nucleotides by DNA polymerase, which moves forwards as the template strands unwind at the replication fork...
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However, the template of the lagging strand is oriented in a 5'>>3' direction, so the lagging strand itself is oriented in the 3'>>5' direction, and hence the DNA polymerase complex must move backwards away from the replication fork. Synthesis of the lagging strand proceeds not continuously, as on the leading strand, but discontinuously in a series of repeated steps...
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