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Issue:

Science

 

Written by:

Julia A

 

Date added:

November 30, 2012

 

Level:

University

 

Grade:

A

 

No of pages / words:

2 / 442

 

Was viewed:

6231 times

 

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Essay content:

The extraction buffer will be used to break up proteins and lipids, making it easier to extract DNA from cells. Once the extraction buffer is added to the crushed up strawberry, it will dissolve lipids, and break the cell wall allowing for easier access to the DNA. Cold alcohol precipitation will be used to extract the DNA from the solution of the buffer, and the strawberry mix...
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Cold alcohol precipitation will be used to extract the DNA from the solution of the buffer, and the strawberry mix. The coldness of the alcohol stops the DNA from breaking about by there hydrogen binds that would break apart with heat, Materials Zip lock bag 1 strawberry 10 mL DNA extraction buffer Coffee filter 1 Large test tube Inoculating loop 20 mL Cold Isopropyl alcohol Paper towels Procedure Place strawberry in Zip lock bag Smash strawberry using fingers and fist (2 minutes minimum) Add 10 mL extraction buffer Mush strawberry in bag for 1 minute Assemble filtration apparatus Pour strawberry slush into filtration allowing to drip in test tube Slowly pour cold alcohol into tube Dip inoculating loop into tube of strawberry extract, and alcohol come in contact Record your results Results First Observation: The liquid from the strawberry slush that has been filtered is red , and is opaque...
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General issues of this essay:
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