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Issue:

Science

 

Written by:

William J

 

Date added:

June 2, 2014

 

Level:

College

 

Grade:

B

 

No of pages / words:

1 / 201

 

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Integrases have also been harnessed for stably inserting foreign genes into bacterial chromosomes in the construction of useful strains. It is therefore important to understand both the mechanism and evolution of integration site usage. The longest studied integrase, from phage lambda, has the following prototypical properties (1): it (i) catalyzes integration and excision of a genetic element, (ii) has one highly preferred integration site in the host chromosome (attB), (iii) recombines segments of identical sequence within attB and the attP region in the element (2,3), and (iv) belongs to the tyrosine recombinase family...
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It is therefore important to understand both the mechanism and evolution of integration site usage. The longest studied integrase, from phage lambda, has the following prototypical properties (1): it (i) catalyzes integration and excision of a genetic element, (ii) has one highly preferred integration site in the host chromosome (attB), (iii) recombines segments of identical sequence within attB and the attP region in the element (2,3), and (iv) belongs to the tyrosine recombinase family...
displayed 300 characters

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