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Plasmid Extraction
Essay specific features
Written by:
Denise H
Date added:
April 2, 2015
Level:
University
Grade:
A
No of pages / words:
2 / 379
Was viewed:
7802 times
Rating of current essay:
Essay content:
pRSG 192 is a recombinant plasmid derived from the chb gene and pUC 19, a 2.7kb engineered plasmid which encodes for ampicillin resistance, a portion of the lac operon and a multiple cloning region . The chb gene exists as a 3.6 kb insert in the mutiple cloning region of pUC 19.
The major goals of Experiment One will be to isolate pRSG 192 from an overnight culture of E...
displayed 300 characters
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coli, amplify a region of the chb gene using PCR, and to map restriction sites within the chb gene using restriction analysis and Southern hybridization.
Methods
Plasmid Isolation
Four microfuge tubes containing cell pellets representing 3.0ml of cells(2 x 1.5ml) from an overnight culture of E. coli were prepared...
displayed 300 characters
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