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Rapid, Long-Range Molecular Haplotyping of

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Issue:

Science

 

Written by:

Ethel N

 

Date added:

May 6, 2013

 

Level:

University

 

Grade:

A

 

No of pages / words:

16 / 4392

 

Was viewed:

1734 times

 

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Essay content:

In the case of thiopurine S-methyltransferase (TPMT), differentiating among TPMT*3A, *3B, and *3C alleles is sometimes necessary for predictive genotyping. Methods: The genomic region including the two SNPs that define TPMT*3A, *3B, and *3C alleles was amplified by long-range PCR. The resulting PCR product was circularized by ligation and haplotyped by allele-specific amplification PCR followed by product identification with hybridization probes...
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The resulting PCR product was circularized by ligation and haplotyped by allele-specific amplification PCR followed by product identification with hybridization probes. Results: Critical points were the long-range PCR conditions, including choice of buffer and primers, optimization of the ligation reaction, and selection of primers that allowed for strict allele-specific amplification in the second-round PCR...
displayed 300 characters

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Preventing Repeat Sex Offenses Cpa'S Range why parrot repeat Long Range Casues Of WWII Long Range Casues Of Wwii Rapid Fire The Rapid Spread of Christianity Brooks Range Molecular Modeling Molecular Modelling Molecular Structures Molecular Switches molecular structure Rapid, Long-Range Molecular Haplotyping of Argument For Keeping Repeat Offenders In Jail
 

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