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Rapid, Long-Range Molecular Haplotyping of

Essay specific features

 

Issue:

Science

 

Written by:

Ethel N

 

Date added:

May 6, 2013

 

Level:

University

 

Grade:

A

 

No of pages / words:

16 / 4392

 

Was viewed:

1738 times

 

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Essay content:

In the case of thiopurine S-methyltransferase (TPMT), differentiating among TPMT*3A, *3B, and *3C alleles is sometimes necessary for predictive genotyping. Methods: The genomic region including the two SNPs that define TPMT*3A, *3B, and *3C alleles was amplified by long-range PCR. The resulting PCR product was circularized by ligation and haplotyped by allele-specific amplification PCR followed by product identification with hybridization probes...
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The resulting PCR product was circularized by ligation and haplotyped by allele-specific amplification PCR followed by product identification with hybridization probes. Results: Critical points were the long-range PCR conditions, including choice of buffer and primers, optimization of the ligation reaction, and selection of primers that allowed for strict allele-specific amplification in the second-round PCR...
displayed 300 characters

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