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Essay

Restriction Endonucleases

Essay specific features

 

Issue:

Science

 

Written by:

Shirley M

 

Date added:

May 28, 2012

 

Level:

University

 

Grade:

A

 

No of pages / words:

5 / 1279

 

Was viewed:

6654 times

 

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Essay content:

Both ends compliment each other, which makes the DNA circular once together. This circular DNA is usually present in an infected bacterial cell. This protects the DNA to be degraded by the cell. In this laboratory experiment, restriction enzymes were used to analyze the DNA of the lambda phage. Restriction enzymes cut DNA in specific locations of the DNA sequence...
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Restriction enzymes cut DNA in specific locations of the DNA sequence. The restriction enzymes used were EcoR1 and Bam H1. Both of these restriction enzymes cut DNA into six fragments. Materials Agarose Gel Electrophoresis Apparatus Gel Comb Gel Mold 100x Triacetate EDTA Buffer with a pH of 8.0 Methylene Blue Stain Microwave Micropipette Ice Bath Water Bath 37?XC (Incubator) Water Bath 70?XC (Incubator) Phage Lambda DNA (uncut) EcoR1- Restriction Enzyme EcoR1 + Bam H1 ?V Mixture of Restriction Enzymes Distilled Water Methods ?? Preparing the DNA samples In this lab experiment, four small tubes were labeled 1-4...
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