Step-Wise Purification and Characterization of a Lactate Dehydrogenase Isozyme from unknown tissue sample of Sus domestica

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Issue:

Science

 

Written by:

Nicholas T

 

Date added:

December 11, 2015

 

Level:

University

 

Grade:

A

 

No of pages / words:

6 / 1539

 

Was viewed:

1348 times

 

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Essay content:

We concluded our sample was from the heart of Sus Domestica. The LDH tetramer found in the heart tissue consists of 4 H isozyme subunits of protein coded by the LDHA gene. 4 different steps of purification were attempted and samples taken at the conclusion of each major step were tested for activity...
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It was found that the 65% cut pellet, the second intermediate, had the highest LDH activity of all purifications. Our SEC purified product was also highly active but second to the 65% CP. 2) Abstract. This should be a brief summary of the major conclusions from your experiments, and should not be more than a paragraph in length...
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