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Western Blotting technique laboratory analysis
Essay specific features
Written by:
Tamra B
Date added:
December 8, 2016
Level:
University
Grade:
A
No of pages / words:
3 / 808
Was viewed:
894 times
Rating of current essay:
Essay content:
Neal Burnette and is a pun on the name Southern blot. Southern blot was a name given by Edwin Southern to a technique he developed for DNA detection.
Western blot analysis has the ability to detect one specific protein in a mixture of any number of proteins while at the same time giving you information about the size of the protein...
displayed 300 characters
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It does not matter whether the protein has been synthesized in vivo or in vitro. However, this method does require the use of a high-quality antibody directed against a desired protein. During western blotting procedures, generally, proteins are solubilized with detergents and reducing agents, and then are separated in polyacrylamide gels...
displayed 300 characters
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